PGK1抗体

+ +

•  

   

     Gel: 10%SDS-PAGE, Lysate: 40 μg, Lane 1-5: A549 cells, hela cells, mouse liver tissue, human liver tissue, hepG2 cells, Primary antibody: P08014(PGK1 Antibody) at dilution 1/850, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 20 seconds    

  
  

•  

   

     The image is immunohistochemistry of paraffin-embedded Human liver cancer tissue using P08014(PGK1 Antibody) at dilution 1/40. (Original magnification: ×200)    

  
  

•  

   

     The image is immunohistochemistry of paraffin-embedded Human brain  tissue using P08014(PGK1 Antibody) at dilution 1/40. (Original magnification: ×200)    

  
  

The phosphoglycerate kinase 1 (PGK1) antibody is a key tool for studying the functional roles of PGK1. a ubiquitously expressed metabolic enzyme critical in glycolysis. PGK1 catalyzes the conversion of 1.3-bisphosphoglycerate to 3-phosphoglycerate, generating ATP during glucose metabolism. Beyond its metabolic function, PGK1 is implicated in diverse cellular processes, including DNA repair, autophagy, and apoptosis, and has been linked to cancer progression, neurodegenerative diseases, and viral infections. Its overexpression in tumors is associated with enhanced glycolysis (the Warburg effect), metastasis, and chemoresistance, making it a potential therapeutic target.

PGK1 antibodies are widely used in research to detect protein expression levels, localization, and post-translational modifications. They enable techniques like Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and flow cytometry, aiding in the exploration of PGK1's roles in disease mechanisms. Commercially available PGK1 antibodies are typically raised in hosts such as rabbits or mice, targeting specific epitopes of human PGK1. Validation for specificity and cross-reactivity is essential, as isoforms or homologous proteins may cause false signals. Recent studies also highlight PGK1's non-canonical roles, such as acting as a protein kinase in hypoxia signaling, underscoring the antibody's utility in cutting-edge research. Its applications extend to biomarker discovery and evaluating therapeutic interventions targeting metabolic pathways.