ARHGEF1抗体

The ARHGEF1 antibody is a crucial tool for studying the Rho guanine nucleotide exchange factor 1 (ARHGEF1), also known as p115RhoGEF, which belongs to the RhoGEF family. ARHGEF1 activates Rho GTPases by catalyzing the exchange of GDP for GTP, primarily regulating RhoA. This protein plays a pivotal role in signaling pathways mediated by G protein-coupled receptors (GPCRs) and receptor tyrosine kinases (RTKs), influencing cellular processes like cytoskeletal reorganization, cell migration, and gene expression. ARHGEF1 interacts with heterotrimeric G proteins (Gα12/13) to transduce signals from extracellular stimuli to intracellular Rho-mediated responses. Dysregulation of ARHGEF1 has been implicated in immune disorders, cancer metastasis, and inflammatory diseases. The ARHGEF1 antibody enables researchers to detect and quantify ARHGEF1 expression in various tissues and cell lines, facilitating investigations into its physiological and pathological roles. It is widely used in techniques such as Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to assess protein localization, expression levels, and interactions. Additionally, the antibody aids in studying ARHGEF1's involvement in signaling cascades, including its crosstalk with other RhoGEFs and GTPases. Its specificity is validated through knockout controls or siRNA-mediated silencing, ensuring reliable experimental outcomes. Understanding ARHGEF1's mechanisms via this antibody provides insights into therapeutic targeting for diseases linked to Rho pathway dysregulation.