NCEH1抗体 新品

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     Gel: 10%SDS-PAGE, Lysate: 40 μg, Lane 1-2: Mouse kidney tissue, Mouse heart tissue, Primary antibody: P07724(NCEH1 Antibody) at dilution 1/1350, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 30 seconds    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using P07724(NCEH1 Antibody) at dilution 1/80. (Original magnification: ×200)    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human brain  tissue using P07724(NCEH1 Antibody) at dilution 1/80. (Original magnification: ×200)    

  
  

The NCEH1 (neutral cholesterol ester hydrolase 1) antibody is a tool used to detect the enzyme NCEH1. which plays a critical role in cellular cholesterol homeostasis. NCEH1. also known as AADACL1 or KIAA1363. belongs to the esterase/lipase protein family and hydrolyzes cholesterol esters into free cholesterol and fatty acids. This activity is vital for regulating intracellular cholesterol levels, impacting membrane structure, steroidogenesis, and lipoprotein metabolism. Dysregulation of NCEH1 has been linked to metabolic disorders, atherosclerosis, neurodegenerative diseases (e.g., Alzheimer's), and cancer progression.

The antibody is widely employed in research to study NCEH1 expression, localization, and function via techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF). It typically targets conserved epitopes across species, including human, mouse, and rat, enabling cross-species studies. Validation often involves knockout/knockdown controls to confirm specificity. Commercially available NCEH1 antibodies may vary in clonality (monoclonal or polyclonal) and conjugation (e.g., HRP, fluorescent tags), tailored to experimental needs.

Understanding NCEH1's role through antibody-based assays contributes to insights into lipid metabolism pathways and therapeutic targeting in diseases associated with cholesterol imbalance. Researchers prioritize antibodies with high sensitivity and minimal cross-reactivity to ensure reliable data in complex biological samples.