SLC12A1抗体 新品

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     Gel: 12%SDS-PAGE, Lysate: 40 μg, Lane: Human fetal kidney tissue, Primary antibody: P07599(SLC12A1 Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human colon cancer tissue using P07599(SLC12A1 Antibody) at dilution 1/15. (Original magnification: ×200)    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human brain tissue using P07599(SLC12A1 Antibody) at dilution 1/15. (Original magnification: ×200)    

  
  

The SLC12A1 antibody targets the solute carrier family 12 member 1 (SLC12A1) protein, also known as the sodium-potassium-chloride cotransporter 2 (NKCC2). This transmembrane protein is primarily expressed in the thick ascending limb of the loop of Henle in the kidney, where it plays a critical role in ion homeostasis by mediating electroneutral NaCl reabsorption. NKCC2 facilitates the coupled transport of Na⁺, K⁺, and Cl⁻ ions across the apical membrane of renal epithelial cells, a process driven by the sodium gradient established by Na⁺/K⁺-ATPase. Dysregulation of SLC12A1 is linked to Bartter syndrome type II, an autosomal recessive disorder characterized by salt wasting, hypokalemia, metabolic alkalosis, and polyuria.

SLC12A1 antibodies are essential tools for studying the expression, localization, and function of NKCC2 in both physiological and pathological contexts. They are widely used in techniques such as Western blotting, immunohistochemistry, and immunofluorescence to investigate renal tubular function, ion transport mechanisms, and genetic or acquired kidney diseases. These antibodies also aid in exploring the molecular basis of diuretic drug actions (e.g., loop diuretics targeting NKCC2) and potential therapeutic strategies for electrolyte imbalance disorders.

Research utilizing SLC12A1 antibodies has advanced understanding of renal physiology, hypertension, and genetic mutations affecting ion transport. Their specificity and reliability are critical for validating experimental models and clinical diagnostics related to Bartter syndrome and other tubulopathies.