PRDX3抗体

+ +

•  

   

     Gel: 10%SDS-PAGE, Lysate: 40 μg, Lane 1-5: Hela cells, 293T cells, MCF7 cells, human fetal brain tissue, Jurkat cells, Primary antibody: P07558(PRDX3 Antibody) at dilution 1/800, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 2 seconds    

  
  

•  

   

     The image is immunohistochemistry of paraffin-embedded Human breast cancer tissue using P07558(PRDX3 Antibody) at dilution 1/50. (Original magnification: ×200)    

  
  

•  

   

     The image is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using P07558(PRDX3 Antibody) at dilution 1/50. (Original magnification: ×200)    

  
  

**Background of PRDX3 Antibody**

Peroxiredoxin-3 (PRDX3), a member of the peroxiredoxin family, is a mitochondrial antioxidant enzyme critical for neutralizing reactive oxygen species (ROS) and maintaining redox homeostasis. It plays a vital role in protecting mitochondrial components from oxidative damage, regulating apoptosis, and modulating cellular proliferation. PRDX3 is encoded by the *PRDX3* gene and is predominantly localized to the mitochondria, distinguishing it from other cytosolic peroxiredoxins.

PRDX3 antibodies are immunological tools designed to detect and quantify PRDX3 protein expression in research applications such as Western blotting (WB), immunohistochemistry (IHC), and immunofluorescence (IF). These antibodies are pivotal in studying PRDX3's involvement in oxidative stress-related pathologies, including neurodegenerative diseases, cancer, cardiovascular disorders, and diabetes. For instance, PRDX3 overexpression has been linked to cancer progression and chemoresistance, while its deficiency correlates with mitochondrial dysfunction and accelerated aging.

Structurally, PRDX3 functions as a homodimer, utilizing cysteine residues to catalyze peroxide reduction. Antibodies targeting PRDX3 often recognize specific epitopes, such as its C-terminal or catalytic domains, enabling researchers to assess its expression levels, subcellular localization, and post-translational modifications. Validation of PRDX3 antibodies is essential to ensure specificity, as cross-reactivity with other peroxiredoxin isoforms (e.g., PRDX1 or PRDX2) can compromise experimental accuracy.

Overall, PRDX3 antibodies are indispensable for exploring mitochondrial oxidative stress mechanisms, disease biomarkers, and therapeutic targets, bridging molecular insights with clinical applications.