PRKCA抗体

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     Gel: 10%SDS-PAGE, Lysate: 50 μg, Lane 1-2: Jurkat cells, HT29 cells, Primary antibody: P07546(PRKCA Antibody) at dilution 1/150, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 20 seconds    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human liver cancer tissue using P07546(PRKCA Antibody) at dilution 1/10. (Original magnification: ×200)    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human lung cancer tissue using P07546(PRKCA Antibody) at dilution 1/10. (Original magnification: ×200)    

  
  

The PRKCA antibody is a crucial tool in biomedical research, specifically targeting the protein kinase C alpha (PKCα) enzyme encoded by the PRKCA gene. PKCα belongs to the protein kinase C (PKC) family of serine/threonine kinases, which play pivotal roles in signal transduction pathways regulating cell proliferation, differentiation, apoptosis, and migration. Activated by calcium and diacylglycerol, PKCα interacts with various cellular components, including membrane phospholipids and secondary messengers, to modulate intracellular signaling cascades such as the MAPK/ERK and PI3K/AKT pathways. Dysregulation of PKCα has been implicated in numerous diseases, including cancer, cardiovascular disorders, and neurological conditions.

PRKCA antibodies are widely utilized to detect and quantify PKCα expression in tissues or cell lines via techniques like Western blotting, immunohistochemistry, and immunofluorescence. They also aid in studying post-translational modifications, subcellular localization, and protein-protein interactions of PKCα under physiological or pathological conditions. Researchers employ these antibodies to explore PKCα's role in disease mechanisms, drug responses, and as a potential therapeutic target. Specific validation steps, such as knockout controls or peptide blocking assays, are essential to ensure antibody specificity due to structural similarities among PKC isoforms. Overall, PRKCA antibodies are indispensable for advancing our understanding of PKCα's multifaceted functions in health and disease.