EIF2AK3抗体 新品

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     Gel: 6%SDS-PAGE, Lysate: 40 μg, Lane: HepG2 cell lysate, Primary antibody: P07537(EIF2AK3 Antibody) at dilution 1/400, Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution, Exposure time: 1 minute    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human breast cancer tissue using P07537(EIF2AK3 Antibody) at dilution 1/60. (Original magnification: ×200)    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human tonsil tissue using P07537(EIF2AK3 Antibody) at dilution 1/60. (Original magnification: ×200)    

  
  

The EIF2AK3 antibody targets the protein EIF2AK3 (Eukaryotic Translation Initiation Factor 2 Alpha Kinase 3), also known as PERK (PKR-like ER kinase). As a key sensor in the unfolded protein response (UPR), PERK is activated during endoplasmic reticulum (ER) stress. It phosphorylates the alpha subunit of eukaryotic initiation factor 2 (eIF2α), temporarily halting global protein synthesis to reduce ER workload while promoting selective translation of stress-related genes. This mechanism aids cellular recovery, but chronic activation can trigger apoptosis, linking EIF2AK3 dysfunction to diseases like diabetes, neurodegenerative disorders, and cancer. Mutations in the EIF2AK3 gene cause Wolcott-Rallison syndrome, a rare autosomal recessive condition characterized by infantile diabetes and multisystem complications.

EIF2AK3 antibodies are essential tools for studying PERK expression, activation (e.g., phosphorylated forms), and localization in cells or tissues. They enable detection via Western blotting, immunofluorescence, and immunohistochemistry, facilitating research into ER stress pathways and disease mechanisms. Commercial EIF2AK3 antibodies are widely used in both basic and translational studies to explore therapeutic targets for conditions involving UPR dysregulation. Validation of antibody specificity remains critical, as cross-reactivity with other eIF2α kinases (e.g., GCN2. PKR) may occur. Overall, these antibodies contribute significantly to understanding cellular stress responses and their pathological implications.