POU2F1抗体

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     Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane: 293T cells, Primary antibody: P07503(POU2F1 Antibody) at dilution 1/400, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 15 seconds    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human colon cancer tissue using P07503(POU2F1 Antibody) at dilution 1/30. (Original magnification: ×200)    

  
  

The POU2F1 antibody is a crucial tool in molecular and cellular biology research, targeting the POU2F1 protein (also known as Oct-1), a member of the POU-domain transcription factor family. POU2F1 plays a pivotal role in regulating gene expression by binding to octamer motifs (ATGCAAAT) in DNA, influencing processes like cell proliferation, differentiation, and apoptosis. It is ubiquitously expressed and interacts with various co-regulators to modulate immune response genes, metabolic pathways, and stress responses. Dysregulation of POU2F1 has been linked to cancers, autoimmune disorders, and viral pathogenesis, as it can either activate or repress target genes depending on cellular context.

Antibodies against POU2F1 are widely used in techniques such as Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and chromatin immunoprecipitation (ChIP) to study its expression, localization, and DNA-binding activity. These antibodies aid in elucidating POU2F1’s role in development, oncogenesis, and immune regulation. Researchers often validate antibody specificity using knockout cells or competitive peptides to ensure accurate detection. Both monoclonal and polyclonal variants are available, with selection depending on experimental requirements. Understanding POU2F1 dynamics through these antibodies continues to advance insights into disease mechanisms and potential therapeutic targets.