MCM8抗体

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     Gel: 10%SDS-PAGE, Lysate: 40 μg, Lane: Jurkat cells, Primary antibody: P07447(MCM8 Antibody) at dilution 1/300, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute    

  
  

The MCM8 antibody is a crucial tool for studying the Minichromosome Maintenance 8 (MCM8) protein, a member of the conserved MCM family involved in DNA replication and repair. MCM8 forms a hexameric complex with MCM9 or interacts with the MCM2-7 helicase complex, playing roles in DNA unwinding during replication initiation and homologous recombination repair. It is essential for maintaining genome stability, particularly during meiosis, and its dysfunction is linked to reproductive disorders, such as premature ovarian insufficiency (POI), and cancer susceptibility due to impaired DNA repair mechanisms.

MCM8 antibodies are widely used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to detect protein expression, localization, and interactions. These antibodies help elucidate MCM8’s tissue-specific roles, particularly in germline development and somatic cell proliferation. Researchers also employ them to investigate MCM8’s association with diseases, including its downregulation in certain cancers (e.g., hepatocellular carcinoma) and mutations in infertility cases.

Commercial MCM8 antibodies are typically raised in rabbits or mice, targeting specific epitopes (e.g., N-terminal or C-terminal regions). Validation for specificity and sensitivity is critical, as cross-reactivity with other MCM proteins may occur. Recent studies highlight MCM8 as a potential biomarker or therapeutic target, driving demand for reliable antibodies in both basic research and clinical diagnostics.