KIF3A抗体

The KIF3A antibody is a crucial tool for studying the KIF3A protein, a member of the kinesin-2 family of motor proteins involved in intracellular transport. KIF3A forms a heterodimer with KIF3B, which associates with the accessory protein KAP3 to create the KIF3 complex. This complex drives anterograde movement of cargo along microtubules, playing essential roles in cilia formation, Hedgehog signaling, and organelle transport. Dysregulation of KIF3A is linked to ciliopathies, neurodegenerative diseases, and cancer. Researchers use KIF3A antibodies in techniques like Western blotting, immunofluorescence, and immunohistochemistry to detect protein expression, localization, and interaction partners. These antibodies help elucidate KIF3A's function in cellular processes, including mitotic spindle organization, neuronal development, and ciliary trafficking. Commercially available KIF3A antibodies are typically raised against specific epitopes (e.g., N-terminal or C-terminal regions) and require validation via knockout cell lines or siRNA-mediated knockdown to confirm specificity. Their applications extend to disease models, particularly in studying ciliopathy mechanisms and cancer cell motility. Proper antibody selection depends on experimental context, as post-translational modifications or splice variants may affect detection.