KLF11抗体

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     Gel: 6%SDS-PAGE, Lysate: 40 μg, Lane 1-4: HUVEC cells, NIH/3T3 cells, Hela cells, 293T cells, Primary antibody: P07405(KLF11 Antibody) at dilution 1/475, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 30 seconds    

  
  

The Krüppel-like factor 11 (KLF11) antibody is a research tool designed to detect and study the KLF11 protein, a member of the Krüppel-like family of zinc finger transcription factors. KLF11. also known as TIEG2. regulates gene expression by binding to GC-rich DNA sequences and recruiting co-repressor complexes like Sin3/HDAC to modulate chromatin structure. It plays roles in cellular processes such as proliferation, differentiation, and apoptosis, with implications in metabolic homeostasis, cancer, fibrosis, and neurodegenerative diseases.

KLF11 antibodies are widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to analyze protein expression, localization, and interactions in tissues or cell lines. Researchers employ these antibodies to explore KLF11's involvement in pathologies; for instance, its downregulation in pancreatic cancer or its regulatory effects on insulin sensitivity in diabetes.

Antibodies targeting KLF11 are typically raised in rabbits or mice, with options for monoclonal or polyclonal formats. Validation includes specificity checks using knockout controls or siRNA-mediated silencing. Commercial KLF11 antibodies often cite applications in studying TGF-β signaling, oxidative stress responses, or epigenetic mechanisms. However, performance may vary depending on epitope regions and experimental conditions, necessitating protocol optimization.

Overall, KLF11 antibodies are critical for elucidating the protein's pathophysiological roles and its potential as a therapeutic target.