IRF9抗体

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     Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane 1-2: Mouse panceas tissue, NIH/3T3 cells, Primary antibody: P07392(IRF9 Antibody) at dilution 1/380, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1.5 minutes    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human tonsil tissue using P07392(IRF9 Antibody) at dilution 1/50. (Original magnification: ×200)    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using P07392(IRF9 Antibody) at dilution 1/50. (Original magnification: ×200)    

  
  

The IRF9 (Interferon Regulatory Factor 9) antibody is a key tool for studying the function of IRF9. a transcription factor critical in mediating interferon (IFN) signaling. IRF9 is a member of the interferon regulatory factor family and forms the ISGF3 (IFN-Stimulated Gene Factor 3) complex with phosphorylated STAT1 and STAT2 upon IFN-α/β stimulation. This complex translocates to the nucleus, binds IFN-stimulated response elements (ISREs), and activates the transcription of interferon-stimulated genes (ISGs) involved in antiviral defense, immune regulation, and cell proliferation. Dysregulation of IRF9 has been linked to autoimmune diseases, viral infections, and cancer, making it a target for therapeutic research.

IRF9 antibodies are widely used in molecular and cellular studies to detect IRF9 expression, localization, and interactions via techniques like Western blotting, immunofluorescence, and co-immunoprecipitation. They are essential for investigating IFN signaling dynamics, JAK-STAT pathway activity, and cellular responses to pathogens or cytokines. Commercially available IRF9 antibodies are typically generated in rabbits or mice using immunogenic peptides corresponding to conserved regions of human or murine IRF9. Validation includes specificity tests using knockout cell lines or siRNA-mediated knockdown. Researchers prioritize antibodies with high affinity, minimal cross-reactivity, and compatibility with their experimental models to ensure reliable data in studies on innate immunity, cancer biology, or inflammatory disorders.