APPL1抗体

+ +

•  

   

     Gel: 10%SDS-PAGE, Lysate: 40 μg, Lane 1-2: SKOV3 cells, K562 cells, Primary antibody: P07118(APPL1 Antibody) at dilution 1/275, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 3 minutes    

  
  

•  

   

     The image is immunohistochemistry of paraffin-embedded Human liver cancer tissue using P07118(APPL1 Antibody) at dilution 1/30. (Original magnification: ×200)    

  
  

•  

   

     The image is immunohistochemistry of paraffin-embedded Human lung cancer tissue using P07118(APPL1 Antibody) at dilution 1/30. (Original magnification: ×200)    

  
  

APPL1 (Adaptor Protein containing PH domain, PTB domain, and Leucine zipper motif 1) is a multifunctional adaptor protein involved in diverse cellular processes, including signal transduction, membrane trafficking, and organelle crosstalk. It interacts with various signaling molecules, such as AKT, Rab5. and Ras family proteins, and plays critical roles in regulating insulin signaling, energy metabolism, cell proliferation, and apoptosis. APPL1 is localized to endosomes, mitochondria, and the nucleus, acting as a scaffold to mediate cross-talk between pathways like the PI3K/AKT and AMPK pathways. Dysregulation of APPL1 has been linked to metabolic disorders (e.g., diabetes, obesity), cancer, and neurodegenerative diseases.

APPL1 antibodies are essential tools for studying its expression, localization, and molecular interactions. They are widely used in techniques such as Western blotting, immunoprecipitation, and immunofluorescence to investigate APPL1's role in cellular processes. Polyclonal and monoclonal antibodies targeting specific domains (e.g., N-terminal or C-terminal regions) enable researchers to probe conformational changes or post-translational modifications. Validated APPL1 antibodies often undergo knockout cell line testing to ensure specificity. These reagents are critical for exploring APPL1's involvement in disease mechanisms, therapeutic target identification, and biomarker discovery. Commercial APPL1 antibodies are typically raised in rabbits or mice, with applications spanning basic research, drug development, and clinical diagnostics.