AMACR抗体

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     Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane: Jurkat cells, Primary antibody: P07073(AMACR Antibody) at dilution 1/350, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 20 seconds    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human liver cancer tissue using P07073(AMACR Antibody) at dilution 1/30. (Original magnification: ×200)    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human gastric cancer tissue using P07073(AMACR Antibody) at dilution 1/30. (Original magnification: ×200)    

  
  

Alpha-methylacyl-CoA racemase (AMACR), also known as P504S, is a mitochondrial and peroxisomal enzyme involved in the β-oxidation of branched-chain fatty acids and bile acid intermediates. Its role in lipid metabolism and association with cancer was first identified in the early 2000s. AMACR gained prominence as a biomarker when studies revealed its overexpression in prostate cancer cells compared to benign prostatic tissue. This discovery positioned AMACR antibodies as valuable tools in diagnostic pathology.

In clinical practice, AMACR immunohistochemical staining is widely used to distinguish prostate adenocarcinoma from benign mimics, particularly in small biopsy specimens. Its utility extends to confirming malignancy in ambiguous glandular proliferations. AMACR is often combined with basal cell markers (e.g., p63. 34βE12) to improve diagnostic accuracy. Beyond prostate cancer, AMACR expression has been observed in other malignancies, including colorectal carcinoma, renal cell carcinoma, and some hepatocellular carcinomas, though with variable specificity.

While highly sensitive for prostate cancer (positive in ~80-95% of cases), AMACR's limitations include occasional negativity in certain subtypes (e.g., foamy or pseudohyperplastic variants) and focal positivity in benign conditions like nephrogenic adenoma. Despite these caveats, AMACR remains a cornerstone in modern pathology workflows, exemplifying the translation of molecular discoveries into practical diagnostic applications.