HSPD1抗体

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     Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane 1-4: NIH/3T3 cell, Hela cell, Mouse fetal tissue, Mouse spleen tissue lysates, Primary antibody: P07358(HSPD1 Antibody) at dilution 1/800, Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution, Exposure time: 1 second    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using P07358(HSPD1 Antibody) at dilution 1/50. (Original magnification: ×200)    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human gastric cancer tissue using P07358(HSPD1 Antibody) at dilution 1/50. (Original magnification: ×200)    

  
  

HSPD1. also known as heat shock protein 60 (HSP60), is a mitochondrial chaperonin belonging to the HSP60 family. It plays a critical role in protein folding, assembly, and quality control within the mitochondrial matrix, particularly under stress conditions. HSPD1 facilitates the proper folding of newly synthesized polypeptides and assists in refolding misfolded proteins, thereby maintaining mitochondrial proteostasis. Dysregulation of HSPD1 is linked to various pathologies, including neurodegenerative diseases, cancer, and autoimmune disorders.

Antibodies targeting HSPD1 are essential tools for studying its expression, localization, and function in cellular and disease contexts. They are widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to detect HSPD1 in tissue samples or cultured cells. In research, HSPD1 antibodies help investigate its role in mitochondrial stress responses, apoptosis, and interactions with other cellular components. Clinically, they aid in exploring HSPD1's potential as a biomarker in cancer prognosis or its involvement in autoimmune conditions like rheumatoid arthritis, where anti-HSP60 autoantibodies may contribute to pathogenesis.

Commercial HSPD1 antibodies are typically raised in hosts like rabbits or mice, with specificity validated using knockout controls. Researchers must consider cross-reactivity with homologous proteins (e.g., bacterial GroEL) and verify antibody performance in specific experimental setups.