GRK4抗体

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     Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane 1-2: 293T and HUVEC cell lysates, Primary antibody: P07329(GRK4 Antibody) at dilution 1/600, Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution, Exposure time: 15 seconds    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human colorectal cancer tissue using P07329(GRK4 Antibody) at dilution 1/60. (Original magnification: ×200)    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human tonsil tissue using P07329(GRK4 Antibody) at dilution 1/60. (Original magnification: ×200)    

  
  

GRK4 (G protein-coupled receptor kinase 4) is a serine/threonine kinase that regulates the desensitization and trafficking of G protein-coupled receptors (GPCRs) by phosphorylating their activated forms. Primarily expressed in the brain, kidneys, and testes, GRK4 plays a critical role in modulating dopamine receptor signaling, particularly in renal proximal tubules, where it influences sodium homeostasis and blood pressure regulation. Genetic variants in *GRK4* (e.g., R65L, A142V, A486V) are linked to salt-sensitive hypertension and altered responses to antihypertensive therapies. Dysregulated GRK4 activity has also been implicated in Parkinson’s disease due to its interaction with dopamine receptor dysfunction.

GRK4 antibodies are essential tools for studying its expression, localization, and molecular mechanisms in both physiological and pathological contexts. These antibodies are widely used in techniques such as Western blotting, immunohistochemistry, and immunofluorescence to quantify GRK4 levels in tissues or cell lines. Commercial GRK4 antibodies are typically raised against specific epitopes (e.g., human GRK4 isoforms α, β, or γ) and validated for species cross-reactivity (human, mouse, rat). Researchers rely on these antibodies to explore GRK4’s role in diseases and its potential as a therapeutic target, particularly for hypertension and neurological disorders. Validation via knockout controls or siRNA knockdown is critical to ensure specificity due to homology among GRK family members.