WNT8A抗体

The Wnt family member 8A (WNT8A) is a secreted glycoprotein involved in the highly conserved Wnt signaling pathway, which plays critical roles in embryonic development, tissue homeostasis, and disease. As a ligand, WNT8A primarily activates the β-catenin-dependent canonical pathway, regulating cell proliferation, differentiation, and patterning during embryogenesis, particularly in neural development and limb formation. WNT8A antibodies are essential tools for studying its expression, localization, and functional interactions. These antibodies are designed to target specific epitopes of the WNT8A protein, enabling detection in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF). Polyclonal antibodies offer broad epitope recognition, while monoclonal antibodies provide high specificity. Validating WNT8A antibodies requires careful testing for cross-reactivity with other Wnt family members (e.g., WNT8B) and confirmation via knockout controls or functional assays. Dysregulation of WNT8A is implicated in developmental disorders and cancers, including colorectal and breast cancer, making its study vital for understanding disease mechanisms. Researchers utilize these antibodies to explore WNT8A's role in stem cell biology, tissue regeneration, and oncogenic signaling, contributing to therapeutic target discovery. Optimal antibody performance depends on sample preparation, fixation methods, and appropriate positive/negative controls to ensure specificity.