WAPL抗体

The WAPL antibody is a crucial tool in studying the WAPL (Wings apart-like) protein, a key regulator of the cohesin complex involved in chromosome organization and sister chromatid cohesion during cell division. WAPL facilitates the release of cohesin from chromosomes, enabling proper chromosome segregation in mitosis and meiosis. Dysregulation of WAPL is linked to genomic instability, developmental disorders, and cancer. Researchers use WAPL antibodies (often rabbit or mouse-derived, polyclonal or monoclonal) in techniques like Western blotting, immunofluorescence, and chromatin immunoprecipitation (ChIP) to investigate its expression, localization, and interactions with partners like PDS5. These studies help elucidate WAPL's roles in DNA repair, transcriptional regulation, and heterochromatin formation. Commercial WAPL antibodies are validated for specificity across human, mouse, and rat models, with applications in cancer biology (e.g., exploring WAPL overexpression in tumors) and developmental research. Recent work also examines WAPL's involvement in 3D genome architecture and epigenetic regulation. Proper experimental controls (e.g., knockout cell lines) are essential to ensure antibody reliability given WAPL's dynamic cell cycle-dependent behavior.