TPST1抗体

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     Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane 1-3: 231 cells, 293T cells, Lovo cells, Primary antibody: P05443(TPST1 Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 2 minutes    

  
  

The tyrosylprotein sulfotransferase 1 (TPST1) antibody is a tool used to study TPST1. an enzyme critical for post-translational protein modification. TPST1 catalyzes tyrosine sulfation—the addition of sulfate groups to tyrosine residues in secretory and membrane proteins. This modification enhances protein-protein and protein-ligand interactions, influencing processes like coagulation, immune response, receptor signaling, and cellular adhesion. Dysregulation of TPST1 has been implicated in diseases such as cancer, inflammatory disorders, and metabolic syndromes.

TPST1 antibodies are essential for detecting and quantifying TPST1 expression in tissues or cell lines, often via techniques like Western blotting, immunohistochemistry, or flow cytometry. Researchers use these antibodies to explore TPST1's role in disease mechanisms, including tumor progression (e.g., colorectal and breast cancers) and immune dysregulation. Additionally, they help validate TPST1's interaction partners and assess its localization within the Golgi apparatus, where the enzyme predominantly resides.

Commercial TPST1 antibodies are typically raised in hosts like rabbits or mice, targeting specific epitopes with validated specificity. Their applications span basic research, drug development, and diagnostic investigations, particularly in understanding how tyrosine sulfation contributes to pathological conditions. However, variability in antibody performance (e.g., cross-reactivity) necessitates careful validation for experimental accuracy.