VMP1抗体

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     POLR3D Antibody (Center) (Cat. #P31295) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the POLR3D antibody detected the POLR3D protein (arrow).    

  
  

VMP1 (Vacuole Membrane Protein 1) is a conserved transmembrane protein initially identified for its role in regulating the formation of autophagosomes, critical structures in cellular autophagy. It localizes to endoplasmic reticulum (ER)-associated membranes and dynamically interacts with other autophagy-related proteins, such as ATG16L1 and WIPI2. to facilitate membrane expansion during autophagosome biogenesis. Beyond autophagy, VMP1 is implicated in maintaining ER morphology, lipid droplet dynamics, and membrane contact sites between the ER and Golgi apparatus. Dysregulation of VMP1 has been linked to diseases including cancer, neurodegenerative disorders, and pancreatitis, where impaired autophagy or ER stress contributes to pathogenesis.

VMP1 antibodies are essential tools for studying these processes. They enable the detection and localization of VMP1 in cells and tissues via techniques like immunofluorescence, Western blotting, and immunoprecipitation. Polyclonal and monoclonal VMP1 antibodies are generated using immunogenic peptide sequences or recombinant protein fragments, often validated for specificity using knockout cell lines. Recent studies employ these antibodies to explore VMP1's involvement in disease mechanisms, drug response, and its potential as a therapeutic target. However, challenges remain in standardizing antibody performance across experimental models, emphasizing the need for rigorous validation in diverse biological contexts.