TRIM71抗体

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     Gel: 6%SDS-PAGE, Lysate: 40 μg, Lane: Mouse brain tissue, Primary antibody: P05416(TRIM71 Antibody) at dilution 1/300, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 20 seconds    

  
  

TRIM71. a member of the tripartite motif (TRIM) protein family, is an RNA-binding E3 ubiquitin ligase implicated in post-transcriptional gene regulation and embryonic development. Structurally, it contains a RING finger domain (mediating ubiquitination), B-box domains, and a coiled-coil region, along with a C-terminal NHL domain crucial for RNA interaction. TRIM71 plays a key role in stem cell maintenance, cellular reprogramming, and tissue regeneration by regulating microRNA (e.g., let-7) activity and mRNA stability. It is essential during early embryogenesis, with knockout studies in mice showing lethal developmental defects.

In disease contexts, TRIM71 is dysregulated in cancers (e.g., hepatocellular carcinoma, glioblastoma) and linked to tumorigenesis through pathways like Wnt/β-catenin and MYC. Its dual role as oncogene or tumor suppressor depends on cellular context. TRIM71 antibodies are critical tools for detecting protein expression, localization, and interaction partners in studies of development, pluripotency, and cancer. They are validated for techniques including Western blotting, immunofluorescence, and immunoprecipitation. High-quality antibodies require specificity testing via knockout controls due to potential cross-reactivity with other TRIM proteins. Research applications span basic mechanisms of RNA-protein interactions to translational studies exploring TRIM71 as a therapeutic target.