PHKG1抗体

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     Gel: 12%SDS-PAGE, Lysate: 40 μg, Lane: PC3 cell lysate , Primary antibody: P09835(ISG15 Antibody) at dilution 1/350, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 10 seconds    

  
  

PHKG1 (Phosphorylase Kinase Gamma 1) is a catalytic subunit of the phosphorylase kinase (PhK) complex, which plays a critical role in glycogen metabolism by activating glycogen phosphorylase. This enzyme facilitates the release of glucose-1-phosphate from glycogen, a key step in energy homeostasis. PHKG1 is highly expressed in skeletal muscle and liver, tissues with high glycogen turnover, and is regulated by calcium/calmodulin signaling. Its activity is essential for coordinating cellular energy demands during stress or physical activity.

PHKG1 antibodies are tools used to detect and study the expression, localization, and function of the PHKG1 protein in biological samples. These antibodies are widely applied in techniques like Western blotting, immunohistochemistry, and immunofluorescence to investigate PHKG1's role in metabolic regulation, cell cycle control, and apoptosis. Dysregulation of PHKG1 has been implicated in metabolic disorders, certain cancers (e.g., hepatocellular carcinoma), and neuromuscular diseases, making it a potential biomarker or therapeutic target.

Researchers also utilize PHKG1 antibodies to explore its interactions within the PhK complex and its tissue-specific isoforms. Validating antibody specificity through knockout controls or siRNA knockdown is crucial due to homology with other kinase subunits. Commercially available PHKG1 antibodies are typically developed in rabbit or mouse hosts, with reactivity confirmed across human, rat, and mouse models. Understanding PHKG1's molecular mechanisms remains vital for elucidating its physiological and pathological contributions.