HES1抗体

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     Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane: Mouse brain tissue lysate, Primary antibody: P04924(HES1 Antibody) at dilution 1/400, Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution, Exposure time: 5 minutes    

  
  

The HES1 antibody is a crucial tool for studying the HES1 protein, a basic helix-loop-helix (bHLH) transcription factor encoded by the *HES1* gene. HES1 acts as a downstream effector of the Notch signaling pathway, playing pivotal roles in developmental processes, including neurogenesis, somitogenesis, and tissue patterning. It functions as a transcriptional repressor by binding to N-box promoter regions of target genes, regulating cell differentiation, proliferation, and fate decisions. Dysregulation of HES1 is implicated in cancers, stem cell maintenance, and diseases like neurological disorders, making it a key focus in developmental biology and oncology research.

HES1 antibodies are widely used to detect and quantify HES1 expression in various applications, including Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and flow cytometry. These antibodies are typically raised in hosts like rabbits or mice, with monoclonal versions offering high specificity. Validated antibodies often demonstrate cross-reactivity across species such as human, mouse, and rat. Commercial suppliers (e.g., Cell Signaling Technology, Abcam) provide antibodies tested for sensitivity and specificity, often verified via knockout controls. Researchers rely on HES1 antibodies to explore its role in cellular dynamics, developmental pathways, and disease mechanisms, particularly in contexts where Notch signaling or stem cell pluripotency is disrupted. Proper validation ensures reliable results in both basic research and clinical studies.