GJC1抗体

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     Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane: MCF7 cells, Primary antibody: P04789(GJC1 Antibody) at dilution 1/1250, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 5 seconds    

  
  

**Background of GJC1 Antibody**

GJC1 (Gap Junction Protein Gamma 1), also known as Connexin 45 (Cx45), is a transmembrane protein that forms gap junctions, specialized intercellular channels enabling direct exchange of ions, metabolites, and signaling molecules between adjacent cells. These channels play critical roles in synchronizing cellular activities, particularly in excitable tissues like the heart, nervous system, and retina. GJC1 is expressed in specific cell types, including cardiomyocytes, neurons, and oligodendrocytes, and is involved in embryonic development, electrical conduction, and tissue homeostasis.

Antibodies targeting GJC1 are essential tools for studying its expression, localization, and function in physiological and pathological contexts. They are widely used in techniques such as immunohistochemistry (IHC), Western blotting, and immunofluorescence to investigate GJC1's role in cardiac arrhythmias, neurodegenerative disorders, and cancer progression. For example, altered GJC1 expression has been linked to impaired cardiac conduction, demyelinating diseases, and tumor metastasis.

Despite their utility, GJC1 antibodies require rigorous validation due to potential cross-reactivity with other connexin family members (e.g., Cx43. Cx30) that share structural homology. Researchers prioritize antibodies with confirmed specificity to ensure accurate interpretation of data, particularly in complex tissues where multiple connexins coexist. Overall, GJC1 antibodies remain pivotal in advancing our understanding of intercellular communication mechanisms and their implications in disease.