IFT74抗体

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     Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane 1-2: HepG2 cells, 293T cells, Primary antibody: P04786(IFT74 Antibody) at dilution 1/1400, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 15seconds    

  
  

The IFT74 antibody is a crucial tool for studying the intraflagellar transport (IFT) system, essential for the assembly and maintenance of cilia and flagella. IFT74 is a core component of the IFT-B complex, which mediates anterograde transport of cargo from the base to the tip of cilia. This protein plays a vital role in ciliogenesis, signaling pathways (e.g., Hedgehog), and sensory functions. Mutations in IFT74 are linked to ciliopathies, a group of disorders affecting multiple organs, including retinal degeneration, renal abnormalities, and skeletal defects.

Researchers use IFT74 antibodies primarily in techniques like Western blotting, immunofluorescence, and immunohistochemistry to visualize protein expression, localization, and dynamics in ciliated cells or tissues. These antibodies help elucidate ciliary defects in disease models and assess IFT complex interactions. Commercial IFT74 antibodies are typically raised in hosts like rabbits or mice, targeting specific epitopes (e.g., N-terminal or C-terminal regions). Validation includes testing in knockout controls or siRNA-treated samples to confirm specificity.

Studies employing IFT74 antibodies have advanced understanding of ciliary biology, contributing to therapeutic insights for ciliopathies and cancer, where disrupted cilia are implicated in tumor progression and metastasis.