ZWILCH 抗体

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     Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane 1-2: HT29 cells, hela cells, Primary antibody: P03646(ZWILCH   Antibody) at dilution 1/1750, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 3 seconds    

  
  

The ZWILCH antibody targets the ZWILCH protein, a component of the mitotic checkpoint complex (MCC) critical for proper chromosome segregation during cell division. ZWILCH, also known as Zwilch kinetochore protein, interacts with other checkpoint proteins like ZWINT and Rod to ensure accurate microtubule-kinetochore attachment. It plays a role in the spindle assembly checkpoint (SAC), delaying anaphase until all chromosomes are correctly aligned, thereby preventing aneuploidy—a hallmark of cancer and genetic disorders.

ZWILCH antibodies are essential tools in studying cell cycle regulation, mitotic errors, and chromosomal instability. They are used in techniques like immunofluorescence, Western blotting, and immunohistochemistry to localize ZWILCH in cells or tissues, assess its expression levels, or analyze its interaction partners. Research has linked aberrant ZWILCH expression to tumorigenesis, making it a potential biomarker or therapeutic target in cancers. Commercial ZWILCH antibodies are typically raised in rabbits or mice, validated for specificity across applications.

Understanding ZWILCH's role through antibody-based studies contributes to insights into mitotic fidelity, cancer mechanisms, and developmental disorders caused by chromosomal missegregation.