ZNRF1抗体

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     Gel: 10%SDS-PAGE, Lysate: 30 μg, Lane: Hela cells, Primary antibody: P03643(ZNRF1 Antibody) at dilution 1/500, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 10 seconds    

  
  

The ZNRF1 (Zinc and Ring Finger 1) antibody is a research tool used to detect and study the ZNRF1 protein, an E3 ubiquitin ligase involved in regulating cellular processes such as protein degradation, membrane receptor trafficking, and signaling pathways. ZNRF1 contains a characteristic RING finger domain, enabling its role in ubiquitination—a post-translational modification that tags proteins for proteasomal degradation or functional modulation. It is particularly implicated in the Wnt signaling pathway by targeting Frizzled receptors for lysosomal degradation, thus acting as a negative regulator of Wnt/β-catenin signaling, which is critical in development, stem cell maintenance, and cancer progression.

Studies highlight ZNRF1's involvement in neuronal development, cancer biology, and metabolic regulation. Dysregulation of ZNRF1 has been linked to tumors, neurodegenerative disorders, and metabolic diseases, making it a potential therapeutic target. The ZNRF1 antibody is widely utilized in techniques like Western blotting, immunohistochemistry, and immunofluorescence to assess protein expression, localization, and interaction partners in cell lines or tissue samples. Researchers also employ it to explore mechanisms underlying ZNRF1-mediated ubiquitination and its crosstalk with pathways like Notch or TGF-β. Available as polyclonal or monoclonal versions, the antibody’s specificity and validation (e.g., knockout validation) are essential for ensuring reliable experimental outcomes in both basic and translational research contexts.