TRIM10抗体

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     Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)    

  
  

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     Western blot analysis using HLA-DRA mouse mAb against Ramos (1), and Raji (2) cell lysate.    

  
  

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     Immunofluorescence analysis of Hela cells using HLA-DRA mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)    

  
  

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     Flow cytometric analysis of MCF-7 cells using HLA-DRA mouse mAb (green) and negative control (red).    

  
  

The TRIM10 antibody is a research tool designed to detect and study TRIM10 (Tripartite Motif-containing protein 10), a member of the TRIM protein family. TRIM proteins are characterized by a conserved N-terminal domain structure, including a RING finger, B-box motifs, and a coiled-coil region, which often confer E3 ubiquitin ligase activity and roles in protein-protein interactions. TRIM10. specifically, is implicated in diverse cellular processes, such as innate immunity, erythroid differentiation, and hematopoiesis. It is expressed primarily in hematopoietic tissues and plays a regulatory role in cell proliferation, apoptosis, and antiviral responses. Studies suggest TRIM10 may interact with intermediate filaments and influence cytoskeletal organization in red blood cells, linking it to disorders like anemia.

The TRIM10 antibody is commonly used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to investigate its expression patterns, subcellular localization, and functional mechanisms. Researchers employ this antibody to explore TRIM10's involvement in diseases, particularly hematologic malignancies, autoimmune conditions, and viral infections. Its specificity and validation (e.g., knockout controls, peptide blocking) are critical to ensure accurate detection, given potential cross-reactivity with other TRIM family members. Current research continues to unravel TRIM10's dual roles in promoting ubiquitination-dependent signaling pathways and its tissue-specific contributions to immune regulation and erythropoiesis.