TFEB抗体

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     Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane 1-2: Jurkat and Hela cell lysates, Primary antibody: P01882(TFEB Antibody) at dilution 1/650, Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution, Exposure time: 90 seconds    

  
  

**Background of TFEB Antibodies**

Transcription factor EB (TFEB) is a member of the MiT/TFE family of basic helix-loop-helix leucine zipper transcription factors, known for regulating lysosomal biogenesis, autophagy, and cellular stress responses. It binds to CLEAR (Coordinated Lysosomal Expression and Regulation) elements in target gene promoters, modulating pathways critical for cellular homeostasis, nutrient sensing, and organelle degradation. Dysregulation of TFEB is linked to diseases, including cancer, neurodegenerative disorders (e.g., Alzheimer’s, Parkinson’s), and lysosomal storage diseases.

TFEB antibodies are essential tools for studying its expression, localization, and function. They enable detection of TFEB in techniques like Western blotting, immunofluorescence, and immunohistochemistry. Specific antibodies can distinguish between cytoplasmic (inactive) and nuclear (active) TFEB, providing insights into its activation status under stress (e.g., starvation, lysosomal dysfunction). Research applications span cancer biology, where TFEB influences tumor progression and drug resistance, and neurology, where its role in clearing protein aggregates is pivotal.

Antibodies targeting TFEB are available as monoclonal or polyclonal variants, often validated for cross-reactivity and species specificity. Their development has advanced understanding of TFEB-mediated pathways, highlighting its therapeutic potential in modulating autophagy-lysosomal activity for disease intervention.