RASSF4抗体

The Ras association domain family member 4 (RASSF4) is a protein belonging to the RASSF tumor suppressor family, which plays critical roles in regulating apoptosis, cell cycle progression, and microtubule stability. RASSF4 is involved in multiple signaling pathways, including the Hippo and Ras pathways, and has been implicated in cancer development, neurodegenerative disorders, and immune responses. RASSF4 antibodies are immunological tools specifically designed to detect and quantify RASSF4 expression in research applications. These antibodies are widely used in techniques such as Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and flow cytometry to study RASSF4's subcellular localization, protein-protein interactions, and expression patterns across tissues. Due to RASSF4's tumor-suppressive functions and epigenetic silencing in certain cancers, its antibodies are valuable for investigating mechanisms of carcinogenesis, therapeutic resistance, and disease biomarkers. Researchers often validate antibody specificity using knockout cell lines or siRNA-mediated RASSF4 knockdown. Commercial RASSF4 antibodies are typically raised in rabbits or mice, targeting specific epitopes within the protein’s N-terminal or Ras-association domain. Proper validation and optimization are essential to ensure reliable results, given potential cross-reactivity with other RASSF family members or isoforms.