NUP85抗体

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     Gel: 6%SDS-PAGE, Lysate: 40 μg, Lane: Hela cells, Primary antibody: P01628(NUP85 Antibody) at dilution 1/300, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute    

  
  

The NUP85 antibody is a crucial tool for studying the nucleoporin 85 kDa (NUP85), a component of the nuclear pore complex (NPC). NUP85 is part of the conserved NUP107-160 subcomplex, which plays a central role in NPC assembly, nucleocytoplasmic transport, and mitotic regulation. This subcomplex contributes to the structural scaffold of the NPC and interacts with other nucleoporins to maintain nuclear envelope integrity. NUP85 itself facilitates protein and RNA trafficking by forming a channel for selective molecular movement between the nucleus and cytoplasm. Dysregulation of NUP85 has been linked to diseases, including primary glomerulopathies and certain cancers, highlighting its importance in cellular homeostasis.

Antibodies targeting NUP85 are widely used in research to investigate NPC architecture, cell cycle dynamics, and disease mechanisms. They enable detection and localization of NUP85 via techniques like Western blotting, immunofluorescence, and immunoprecipitation. Validating these antibodies for specificity is critical, as cross-reactivity with other nucleoporins can occur due to structural similarities within the NPC. Recent studies also utilize NUP85 antibodies to explore its role in viral infection processes, as some pathogens exploit NPC components for nuclear entry. Overall, NUP85 antibodies serve as essential reagents for dissecting NPC function and its implications in health and disease.