NUMB抗体

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     Gel: 6%SDS-PAGE, Lysate: 40 μg, Lane 1-6: K562 cells, hela cells, skov3 cells, NIH/3T3 cells, Raji cells, human testis tissue, Primary antibody: P01607(NUMB Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 2 minutes    

  
  

The NUMB protein, encoded by the *NUMB* gene, is a key regulator of cell fate determination, initially identified for its role in asymmetric cell division during development. It functions as a tumor suppressor by inhibiting Notch signaling, a pathway critical for cell differentiation and proliferation. NUMB achieves this through its phosphotyrosine-binding (PTB) domain, which mediates interactions with membrane receptors and intracellular partners, and its proline-rich region (PRR), involved in protein-protein interactions. Selective splicing generates multiple NUMB isoforms with distinct functional properties, further modulated by post-translational modifications like phosphorylation and ubiquitination.

NUMB antibodies are essential tools for studying its expression patterns, subcellular localization, and interactions in physiological and pathological contexts. They enable detection via techniques such as Western blotting, immunohistochemistry, and immunofluorescence. Dysregulation of NUMB has been linked to cancers (e.g., breast, lung), neurodegenerative diseases, and developmental disorders, making these antibodies vital for mechanistic research. Commercial NUMB antibodies vary in specificity, often targeting epitopes in conserved regions (e.g., PTB domain) or isoform-unique sequences. Validation across applications and species remains critical due to NUMB's structural complexity and cross-reactivity risks. Recent studies also highlight NUMB's role in endocytosis, cell polarity, and stem cell maintenance, expanding its relevance in biomedical research.