P3H3抗体

+ +

•  

   

     Gel: 6%SDS-PAGE, Lysate: 40 μg, Lane 1-3: A549 cells, mouse heart tissue, Hela cells, Primary antibody: P01512(P3H3 Antibody) at dilution 1/300, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 20 seconds    

  
  

•  

   

     The image is immunohistochemistry of paraffin-embedded Human rectal cancer tissue using P01512(P3H3 Antibody) at dilution 1/50. (Original magnification: ×200)    

  
  

•  

   

     The image is immunohistochemistry of paraffin-embedded Human placenta tissue using P01512(P3H3 Antibody) at dilution 1/50. (Original magnification: ×200)    

  
  

The P3H3 antibody targets the prolyl 3-hydroxylase 3 (P3H3) enzyme, a member of the prolyl hydroxylase family involved in collagen biosynthesis. P3H3. encoded by the *LEPREL1* gene, catalyzes the post-translational hydroxylation of specific proline residues in collagen chains, a critical modification for collagen triple-helix stability and extracellular matrix integrity. While P3H1 and P3H2 are well-studied in collagen IV and fibrillar collagen modification, respectively, P3H3’s precise biological role remains less defined, though it is implicated in collagen VI and IX processing.

Antibodies against P3H3 are primarily used in research to investigate its expression, localization, and function in tissues, particularly in connective tissues and organs rich in collagen, such as skin, bone, and cartilage. Studies suggest P3H3 dysregulation may contribute to connective tissue disorders, fibrosis, or cancer progression, making it a potential biomarker or therapeutic target. Commercial P3H3 antibodies are typically validated for applications like Western blotting, immunohistochemistry, and immunofluorescence.

However, challenges exist, including cross-reactivity with other P3H isoforms and variability in antibody performance across species or experimental conditions. Recent advances in epitope mapping and recombinant protein techniques have improved antibody specificity. Ongoing research aims to clarify P3H3’s mechanistic role in collagen maturation and disease, supported by tools like P3H3 antibodies to bridge structural biology with clinical insights.