GLRX抗体

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     Gel: 12%SDS-PAGE, Lysate: 40 μg, Lane: Human placenta tissue lysate, Primary antibody: P01412(GLRX Antibody) at dilution 1/400, Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution, Exposure time: 40 seconds    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human liver cancer tissue using P01412(GLRX Antibody) at dilution 1/50. (Original magnification: ×200)    

  
  

The glutaredoxin (GLRX) family comprises small, evolutionarily conserved redox enzymes critical for maintaining cellular redox homeostasis. These proteins, typically 10-12 kDa, belong to the thioredoxin superfamily and utilize a conserved cysteine-containing active site (CXXC motif) to catalyze thiol-disulfide exchange reactions. GLRX proteins primarily function in reducing glutathione-mixed disulfides, regenerating oxidized substrates, and regulating protein glutathionylation—a post-translational modification influencing protein activity and signaling pathways.

Three main isoforms (GLRX1. GLRX2. GLRX3) exist in mammals, with distinct subcellular localizations: GLRX1 is cytosolic, GLRX2 mitochondrial, and GLRX3 nuclear/cytosolic. Their roles span iron-sulfur cluster biosynthesis, antioxidant defense, and modulation of redox-sensitive transcription factors (e.g., NF-κB). Dysregulation of GLRX expression correlates with pathological conditions, including cancer progression, neurodegenerative disorders (e.g., Parkinson's), cardiovascular diseases, and diabetes, highlighting their involvement in oxidative stress responses.

GLRX antibodies are essential tools for detecting and quantifying these proteins in techniques like Western blotting, immunohistochemistry, and ELISA. They enable researchers to investigate GLRX expression patterns in disease models, assess subcellular distribution, and study interactions with client proteins. Commercial antibodies often target isoform-specific regions, aiding functional studies of individual GLRX members. Validated GLRX antibodies are crucial for advancing research into redox biology and developing therapeutic strategies targeting oxidative stress-related diseases.