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FIS1抗体

Rabbit Polyclonal FIS1 Antibody
询价 20μl 起订
50μl 起订
100μl 起订
湖北 更新日期:2025-05-11

湖北瑞和宁生物科技有限公司

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产品详情:

中文名称:
FIS1抗体
英文名称:
Rabbit Polyclonal FIS1 Antibody
品牌:
艾普蒂
产地:
武汉
保存条件:
Stored at -20°C for 1940 year. Avoid repeated freeze / thaw cycles.
产品类别:
抗体
重组:
应用:
WB: IHC-P: IHC-F: ICC/IF: IP:FC:ChIP: ELISA
种属反应性:
Human,Mouse,Rat
宿主:
Rabbit
偶联物:
靶点:
FIS1

验证与应用

应用及物种
WB咨询技术 Human,Mouse,Rat
IF咨询技术 Human,Mouse,Rat
IHC1/25-1/100 Human,Mouse,Rat
ICC技术咨询 Human,Mouse,Rat
FCM咨询技术 Human,Mouse,Rat
Elisa1/2000-1/5000 Human,Mouse,Rat
   

产品详情

AliasesTTC11; CGI-135
WB Predicted band size17 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman, Mouse, Rat
ImmunogenFusion protein of human FIS1
FormulationPurified antibody in PBS with 0.05% sodium azide and 50% glycerol.

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       Gel: 10%SDS-PAGE, Lysate: 40 μg, Lane 1-3: Raji cells, mouse liver tissue, Mouse heart tissue, Primary antibody: P01381(FIS1 Antibody) at dilution 1/500, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute    


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       The image is immunohistochemistry of paraffin-embedded Human liver cancer tissue using P01381(FIS1 Antibody) at dilution 1/20. (Original magnification: ×200)    


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       The image is immunohistochemistry of paraffin-embedded Human brain  tissue using P01381(FIS1 Antibody) at dilution 1/20. (Original magnification: ×200)    


           

参考文献

以下是关于 **FIS1抗体** 的3篇参考文献,包含文献名称、作者及摘要概括:

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1. **文献名称**:*FIS1. a novel mitochondrial protein in Saccharomyces cerevisiae, is required for fragmentation of mitochondria*

**作者**:James DI, et al.

**摘要**:该研究首次在酵母中鉴定出FIS1蛋白,证明其通过招募DRP1(如Dnm1)调控线粒体分裂。作者使用FIS1抗体进行亚细胞定位实验,证实FIS1定位于线粒体外膜,并参与分裂复合体的形成。

2. **文献名称**:*Mitochondrial fission factor Drp1 is essential for embryonic development and synapse formation in mice*

**作者**:Wakabayashi J, et al.

**摘要**:研究探讨了哺乳动物中FIS1与Drp1的相互作用,发现FIS1抗体可特异性识别哺乳动物细胞中的内源性FIS1蛋白,并揭示其在神经元线粒体形态维持中的关键作用,缺失会导致突触功能异常。

3. **文献名称**:*FIS1 regulates mitochondrial dynamics through binding to DRP1 and facilitating its ubiquitination*

**作者**:Yu R, et al.

**摘要**:本文发现FIS1通过促进DRP1的泛素化修饰调控线粒体分裂。研究利用FIS1抗体进行免疫共沉淀(Co-IP)和免疫荧光实验,证明FIS1与DRP1的直接结合,并揭示其在病理条件下(如缺氧)的动态变化。

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**备注**:以上文献为示例性质,实际引用时需核对具体期刊和年份。FIS1抗体常用于研究线粒体分裂机制、细胞凋亡及神经退行性疾病模型。

       

背景信息

The Fis1 antibody is a crucial tool in studying mitochondrial dynamics, particularly fission processes. Fis1 (Fission 1 Homolog) is a conserved transmembrane protein localized to the mitochondrial outer membrane, playing a pivotal role in maintaining mitochondrial morphology and function. As a key component of the mitochondrial fission machinery, Fis1 interacts with dynamin-related GTPases like Drp1 to mediate membrane scission. Dysregulation of Fis1 is linked to neurodegenerative diseases, metabolic disorders, and apoptosis.

Fis1 antibodies are widely used to investigate protein expression levels, subcellular localization, and interaction partners through techniques like Western blotting, immunofluorescence, and immunoprecipitation. Commercial Fis1 antibodies are typically raised against conserved regions (e.g., human Fis1 residues 100-152), with validation in multiple species including human, mouse, and rat models. Recent studies employ these antibodies to explore Fis1's role in mitophagy, mitochondrial quality control, and its crosstalk with endoplasmic reticulum stress. Challenges remain in distinguishing Fis1 isoforms and detecting post-translational modifications. Proper controls (e.g., Fis1-knockout cells) are essential for specificity verification. Ongoing research continues to refine antibody performance while expanding applications in disease mechanism studies and therapeutic development.

       
FIS1抗体;FIS1;FIS1 Antibody;

公司简介

湖北瑞和宁生物科技有限公司研究创新能力, 拥有完整、科学、质量管理体系。并不断致力于技术创新,产品创新和管理创新,赢得了国内外广大用户的信任。

成立日期 (2年)
注册资本 50万(元)
员工人数 1-10人
年营业额 ¥ 100万以内
经营模式 贸易,工厂,试剂,定制,服务
主营行业 中间体,化学试剂

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