CHMP1A抗体

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     All lanes : Anti-PENK Antibody at 1:2000 dilution Lane 1:  T47D whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Mouse IgG/A/M(H/L),  Peroxidase conjugated   at 1/2000 dilution. Observed band size : 35kDa Blocking/Dilution buffer: 5% NFDM/TBST.    

  
  

CHMP1A (Charged Multivesicular Body Protein 1A) is a key component of the Endosomal Sorting Complex Required for Transport (ESCRT) machinery, which plays essential roles in membrane remodeling processes, including multivesicular body (MVB) formation, cytokinesis, viral budding, and autophagy. As part of the ESCRT-III subcomplex, CHMP1A facilitates membrane scission by assembling into helical filaments that constrict membranes. Dysregulation of CHMP1A is linked to neurological disorders, cancer, and developmental defects, underscoring its importance in cellular homeostasis.

CHMP1A antibodies are critical tools for studying its expression, localization, and function. These antibodies are commonly used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to detect CHMP1A in various biological samples. Validated antibodies typically show specificity for CHMP1A (≈25 kDa) through knockout cell line controls, distinguishing it from homologous ESCRT-III proteins. Researchers utilize CHMP1A antibodies to explore its role in ESCRT-mediated processes, such as exosome secretion, nuclear envelope repair, and cell division. Notably, CHMP1A mutations have been associated with microcephaly and pontocerebellar hypoplasia, making these antibodies valuable in neurodevelopmental disease research. Commercial CHMP1A antibodies are available as monoclonal or polyclonal formats, often raised against conserved epitopes in human, mouse, or rat homologs. Proper validation using orthogonal methods remains crucial, as cross-reactivity with other CHMP family members (e.g., CHMP1B) can occur. Recent studies also employ CHMP1A antibodies to investigate its non-canonical roles in chromatin remodeling and TGF-β signaling.