NAE1抗体

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     The image is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using P00942(NAE1 Antibody) at dilution 1/20. (Original magnification: ×200)    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human lung cancer tissue using P00942(NAE1 Antibody) at dilution 1/20. (Original magnification: ×200)    

  
  

**Background of NAE1 Antibody**

NAE1 (NEDD8-Activating Enzyme E1), also known as APPBP1 or UBA3-interacting protein, is a critical subunit of the NEDD8-activating enzyme complex. This enzyme plays a central role in the NEDDylation pathway, a post-translational modification process analogous to ubiquitination. NAE1. in conjunction with UBA3. forms the heterodimeric E1 enzyme responsible for activating NEDD8 (Neural Precursor Cell Expressed Developmentally Downregulated Protein 8), a ubiquitin-like protein. The NAE1-UBA3 complex catalyzes ATP-dependent NEDD8 transfer to E2 conjugating enzymes, ultimately facilitating the attachment of NEDD8 to target proteins, such as cullin family members. This modification regulates the activity of cullin-RING ubiquitin ligases (CRLs), which are essential for protein degradation and cellular processes like cell cycle progression, DNA repair, and signal transduction.

NAE1 antibodies are valuable tools for studying the molecular mechanisms of NEDDylation and its implications in diseases, particularly cancer. Dysregulation of NAE1 and hyperactivation of NEDDylation are linked to tumorigenesis, making NAE1 a therapeutic target. Inhibitors like MLN4924 (Pevonedistat), which blocks NAE1 activity, are under clinical investigation. Researchers use NAE1 antibodies in techniques such as Western blotting, immunoprecipitation, and immunohistochemistry to assess protein expression, localization, and interaction partners in both normal and pathological contexts. These studies contribute to understanding cellular homeostasis, oncogenesis, and potential treatment strategies targeting the NEDD8 pathway.