ANKRD1抗体

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     Gel: 8%SDS-PAGE, Lysate: 60 μg, Lane 1-3: Mouse lung tissue, NIH/3T3 cells, hepg2 cells, Primary antibody: P00925(ANKRD1 Antibody) at dilution 1/400, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 3 seconds    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human breast cancer tissue using P00925(ANKRD1 Antibody) at dilution 1/20. (Original magnification: ×200)    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using P00925(ANKRD1 Antibody) at dilution 1/20. (Original magnification: ×200)    

  
  

The ANKRD1 antibody is a valuable tool for studying the ANKRD1 protein (Ankyrin Repeat Domain-Containing Protein 1), also known as CARP (Cardiac Ankyrin Repeat Protein). ANKRD1 is a stress-inducible nuclear and sarcomeric protein predominantly expressed in cardiac and skeletal muscle. It functions as a transcriptional co-regulator involved in muscle development, mechanotransduction, and adaptive responses to mechanical or hypoxic stress. Structurally, it contains an N-terminal nuclear localization signal, ankyrin repeats for protein-protein interactions, and a C-terminal nuclear export signal.

Antibodies targeting ANKRD1 are widely used in Western blotting (WB), immunohistochemistry (IHC), immunofluorescence (IF), and co-immunoprecipitation (Co-IP) to investigate its expression, subcellular localization, and interactions with partners like titin or transcription factors (e.g., YB-1). Research applications span cardiac hypertrophy, heart failure, muscular dystrophy, and cancer, where ANKRD1 exhibits dual roles as a tumor suppressor or promoter depending on context.

Commercial ANKRD1 antibodies are typically monoclonal or polyclonal, raised against epitopes within the ankyrin repeat or C-terminal regions. However, variability in specificity between clones necessitates validation using knockout/knockdown controls. Recent studies highlight its role in fibrosis, apoptosis, and TGF-β signaling, underscoring its therapeutic potential. When selecting an ANKRD1 antibody, consider species reactivity (human, mouse, rat), validated applications, and published data supporting its performance in your experimental model.