OCLN抗体

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     Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane: Mouse liver tissue lysate, Primary antibody: P00583(OCLN Antibody) at dilution 1/550, Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution, Exposure time: 30 seconds    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using P00583(OCLN Antibody) at dilution 1/70. (Original magnification: ×200)    

  
  

Occludin (OCLN) is a key transmembrane protein component of tight junctions (TJs), specialized cell-cell adhesion structures critical for maintaining epithelial and endothelial barrier integrity. First identified in 1993. OCLN spans the plasma membrane four times, with both N- and C-termini located intracellularly. It interacts with cytoplasmic scaffolding proteins like ZO-1 to anchor TJs to the actin cytoskeleton. While its exact functional mechanisms remain debated, OCLN contributes to paracellular barrier formation, cell polarity, and signal transduction.

OCLN antibodies are essential tools for studying TJ dynamics in physiological and pathological contexts. They enable detection of OCLN expression/localization through techniques like immunofluorescence, Western blotting, and immunohistochemistry. Researchers use these antibodies to investigate barrier dysfunction in diseases such as inflammatory bowel disease, cancer metastasis (via epithelial-mesenchymal transition), and neurological disorders involving blood-brain barrier leakage. Species-specific and isoform-targeting antibodies (e.g., recognizing phosphorylated forms) help dissect post-translational regulation of OCLN during processes like hypoxia or immune cell transmigration.

Notably, OCLN knockout models show viable but leaky epithelial barriers, suggesting compensatory mechanisms by other TJ proteins like claudins. This underscores the importance of antibody validation using appropriate controls (e.g., knockout tissues) to ensure specificity. Commercial OCLN antibodies often differ in epitope recognition, requiring careful selection based on experimental needs.