GRB14抗体

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     Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane: Human fetal intestines tissue, Primary antibody: P00405(GRB14 Antibody) at dilution 1/350, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 20 seconds    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human breast cancer tissue using P00405(GRB14 Antibody) at dilution 1/35. (Original magnification: ×200)    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using P00405(GRB14 Antibody) at dilution 1/35. (Original magnification: ×200)    

  
  

The GRB14 antibody is a crucial tool for studying the Growth Factor Receptor-Bound Protein 14 (GRB14), an adaptor protein involved in regulating receptor tyrosine kinase (RTK) signaling pathways. GRB14 belongs to the GRB7 protein family and plays a role in modulating insulin receptor signaling, cell proliferation, and metabolic processes. It interacts with activated insulin receptors and other RTKs, often acting as a negative regulator to fine-tune downstream signaling cascades like the MAPK and PI3K/AKT pathways. Dysregulation of GRB14 has been linked to insulin resistance, type 2 diabetes, obesity, and certain cancers, making it a target for metabolic and oncological research.

The GRB14 antibody is widely used in techniques such as Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and co-immunoprecipitation (Co-IP) to detect GRB14 expression, localization, and protein interactions in tissues or cell lines. Researchers rely on its specificity to investigate GRB14's tissue-specific roles, particularly in insulin-sensitive tissues (e.g., liver, muscle, adipose) and tumors. Commercially available antibodies are typically raised in rabbits or mice, with validation including knockout cell line controls or peptide blocking assays. Its applications extend to studying molecular mechanisms in metabolic disorders, cancer progression, and potential therapeutic targeting of GRB14-associated pathways.