GNB5抗体

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     The image is immunohistochemistry of paraffin-embedded Human liver cancer tissue using P13221(CYB5R3 Antibody) at dilution 1/50. (Original magnification: ×200)    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human gastric cancer tissue using P13221(CYB5R3 Antibody) at dilution 1/50. (Original magnification: ×200)    

  
  

The GNB5 antibody is a crucial tool for studying the β5 subunit of heterotrimeric G proteins, which are essential for intracellular signal transduction. GNB5. encoded by the GNB5 gene, is a member of the Gβ subunit family and plays a unique role in modulating G protein-coupled receptor (GPCR) signaling pathways. Unlike other Gβ subunits, GNB5 preferentially interacts with regulators of G protein signaling (RGS) proteins, particularly those in the R7 subfamily (e.g., RGS6. RGS7), to form complexes that accelerate GTPase activity of Gα subunits, thereby terminating GPCR signals. This interaction is vital for regulating neuronal, cardiovascular, and metabolic functions.

GNB5 antibodies are widely used in research to detect and quantify GNB5 expression in tissues or cells via techniques like Western blotting, immunohistochemistry, and immunofluorescence. They help elucidate GNB5's tissue-specific roles, particularly in the brain, heart, and retina. Mutations in GNB5 are linked to neurodevelopmental disorders, cardiac arrhythmias, and vision impairments, making these antibodies valuable for studying disease mechanisms.

Most commercial GNB5 antibodies are raised against conserved epitopes, often in humans, mice, or rats, and are validated for specificity using knockout controls. Researchers rely on them to explore GNB5's interactions with RGS proteins, its regulatory effects on ion channels, and its broader implications in cellular homeostasis. Proper validation remains critical due to potential cross-reactivity with other Gβ subunits.