E2F6抗体

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     Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane 1-5: LoVo cells, A549 cells, human hepatocellular carcinoma tissue, Jurkat cells, Hela cells, Primary antibody: P00344(E2F6 Antibody) at dilution 1/650, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 30 seconds    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using P00344(E2F6 Antibody) at dilution 1/60. (Original magnification: ×200)    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using P00344(E2F6 Antibody) at dilution 1/60. (Original magnification: ×200)    

  
  

The E2F6 antibody is a crucial tool for studying the E2F transcription factor family, particularly E2F6. a member known for its role in transcriptional repression. E2F proteins regulate cell cycle progression, differentiation, and apoptosis by controlling the expression of genes involved in DNA replication and repair. Unlike activating E2Fs (E2F1-E2F3), E2F6 lacks a transactivation domain and functions primarily as a repressor by forming complexes with polycomb group proteins or other transcriptional inhibitors (e.g., BM1. L3MBTL2). This unique mechanism allows E2F6 to silence target genes during the G1 phase, maintaining quiescence or balancing proliferation in specific cell types.

E2F6 antibodies are widely used in molecular biology to detect and quantify E2F6 protein levels via techniques like Western blotting, immunofluorescence, and chromatin immunoprecipitation (ChIP). Their specificity enables researchers to explore E2F6's involvement in developmental processes, stem cell maintenance, and tumorigenesis. Dysregulation of E2F6 has been linked to cancers, including leukemia and solid tumors, where its overexpression may suppress pro-apoptotic genes or promote unchecked proliferation. Additionally, studies suggest E2F6's role in metabolic regulation and stress responses, expanding its relevance beyond cell cycle control. Validated antibodies with minimal cross-reactivity to other E2F members are essential for accurate experimental outcomes, making E2F6 antibodies a key reagent in both basic and translational research contexts.