DOK3抗体

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     Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane 1-2: A375 and Hela cell lysates, Primary antibody: P13499(LDHA Antibody) at dilution 1/400, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 10 seconds    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human breast cancer tissue using P13499(LDHA Antibody) at dilution 1/40. (Original magnification: ×200)    

  
  

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     The image is immunohistochemistry of paraffin-embedded Human liver cancer tissue using P13499(LDHA Antibody) at dilution 1/40. (Original magnification: ×200)    

  
  

The DOK3 (Downstream of Tyrosine Kinase 3) antibody is a tool used to study the DOK3 protein, a member of the DOK family of adaptor proteins involved in signal transduction. DOK proteins act as scaffolds, modulating intracellular signaling pathways, particularly those downstream of receptor tyrosine kinases (RTKs) and immune receptors. DOK3. primarily expressed in immune cells such as B cells, macrophages, and dendritic cells, plays a regulatory role in immune responses by negatively regulating signaling pathways like the B-cell receptor (BCR) and Toll-like receptor (TLR) cascades. It interacts with signaling molecules, including phosphatases (e.g., SHP-1) and kinases, to dampen activation signals, thereby maintaining immune homeostasis.

DOK3 antibodies are essential for detecting and quantifying DOK3 expression in various experimental settings, including Western blotting, immunohistochemistry, and flow cytometry. Researchers use these antibodies to explore DOK3's role in diseases such as autoimmune disorders, cancers, and inflammatory conditions, where dysregulated signaling pathways are implicated. For example, DOK3 deficiency or dysfunction has been linked to hyperactive immune responses and oncogenic signaling. The antibody's specificity and reliability are critical for elucidating DOK3's molecular interactions and its potential as a therapeutic target. Commercial DOK3 antibodies are typically validated for cross-reactivity across species (e.g., human, mouse) and application-specific performance.