ASF1A抗体

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     Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)    

  
  

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     Flow cytometric analysis of Hela cells using ASF1A mouse mAb (green) and negative control (red).    

  
  

The ASF1A (Anti-silencing Function 1A) antibody targets the ASF1A protein, a conserved histone chaperone critical for chromatin assembly and epigenetic regulation. ASF1A, along with its paralog ASF1B, facilitates the deposition of histones H3 and H4 onto DNA during replication and repair. It plays a key role in replication-coupled nucleosome assembly by interacting with replication protein A (RPA) and the chromatin assembly factor 1 (CAF-1) complex. ASF1A also participates in transcription regulation, DNA damage repair, and cell cycle progression, particularly during S phase. Unlike ASF1B, ASF1A is predominantly involved in replication and repair processes.

ASF1A antibodies are widely used in research to study chromatin dynamics, replication stress responses, and epigenetic modifications. They enable detection of ASF1A expression and localization via techniques like Western blotting, immunofluorescence, and immunohistochemistry. Dysregulation of ASF1A is linked to cancers, where its overexpression correlates with poor prognosis, suggesting its potential as a therapeutic target. These antibodies are essential tools for elucidating ASF1A’s role in genome stability, cellular proliferation, and disease mechanisms.