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  • ATG3抗体

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ATG3抗体

Mouse Monoclonal ATG3 Antibody
询价 20μl 起订
50μl 起订
100μl 起订
湖北 更新日期：2025-05-10

湖北瑞和宁生物科技有限公司

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联系人：周经理
电话：027-19371278702拨打
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邮箱：19384116026@163.com

产品详情:

中文名称：
ATG3抗体
英文名称：
Mouse Monoclonal ATG3 Antibody
品牌：
艾普蒂
产地：
武汉
保存条件：
Stored at -20°C for 950 year. Avoid repeated freeze / thaw cycles.
产品类别：
抗体
重组：
否
应用：
WB: IHC-P: IHC-F: ICC/IF: IP:FC:ChIP: ELISA
种属反应性：
Human,Mouse,Rat
宿主：
Mouse
偶联物：
无
靶点：
ATG3

验证与应用

应用及物种
WB1/500 - 1/2000 Human,Monkey
IF咨询技术 Human,Monkey
IHC1/200 - 1/1000 Human,Monkey
ICC1/200 - 1/1000 Human,Monkey
FCM咨询技术 Human,Monkey
Elisa1/10000 Human,Monkey
   

产品详情

AliasesAPG3; APG3L; PC3-96; APG3-LIKE
Entrez GeneID64422
clone7A1D1
WB Predicted band size35.9kDa
Host/IsotypeMouse IgG1
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman,Monkey
ImmunogenPurified recombinant fragment of human ATG3 (AA: 1-100) expressed in E. Coli.
FormulationPurified antibody in PBS with 0.05% sodium azide

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       Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)    


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       Western blot analysis using ATG3 mouse mAb against Jurkat (1), K562 (2), Hela (3), THP-1 (4), and COS7 (5) cell lysate.    


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       Immunofluorescence analysis of Hela cells using ATG3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)    


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       Immunofluorescence analysis of SMMC-7721 cells using ATG3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)    


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       Immunohistochemical analysis of paraffin-embedded human breast cancer tissues using ATG3 mouse mAb with DAB staining.    


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       Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissues using ATG3 mouse mAb with DAB staining.    


           

参考文献

以下是3-4条关于ATG3抗体的参考文献及其摘要概括：

1. **文献名称**: "Autophagosome formation requires ATG3-dependent conjugation of ATG8 to single membranes"

**作者**: Sou et al.

**摘要**: 研究通过ATG3抗体验证ATG3在自噬体形成中的关键作用，发现ATG3介导ATG8(LC3)与单层膜的脂化，对抗体在免疫印迹和免疫荧光中的应用进行了验证。

2. **文献名称**: "ATG3 regulates mitochondrial homeostasis and protects against neurodegeneration"

**作者**: Tang et al.

**摘要**: 利用ATG3特异性抗体揭示ATG3在维持线粒体功能中的作用，发现其缺失导致神经退行性病变，抗体用于小鼠脑组织样本的Western blot和免疫组化分析。

3. **文献名称**: "A novel ATG3-binding protein promotes autophagy by enhancing LC3 lipidation"

**作者**: Li et al.

**摘要**: 通过免疫共沉淀(使用ATG3抗体)鉴定出ATG3的新型互作蛋白，证明其通过促进ATG8脂化增强自噬活性，抗体在蛋白互作研究中发挥关键作用。

4. **文献名称**: "Antibody-based validation of ATG3 as a therapeutic target in breast cancer"

**作者**: Chen et al.

**摘要**: 研究利用商业化ATG3抗体分析乳腺癌患者样本，发现ATG3高表达与预后不良相关，抗体应用于流式细胞术和免疫组织化学，支持其作为治疗靶点的潜力。

这些文献均涉及ATG3抗体的实验应用(如Western blot、免疫荧光、免疫共沉淀等)，并围绕自噬机制或疾病模型展开研究。

       

背景信息

**Background of ATG3 Antibody**

ATG3 (Autophagy-related protein 3) is a key enzyme in the autophagy pathway, a conserved cellular process critical for degrading damaged organelles, proteins, and pathogens. ATG3 functions as an E2 ubiquitin-conjugating enzyme, facilitating the conjugation of LC3 (Microtubule-associated protein 1A/1B-light chain 3) to phosphatidylethanolamine (PE) during autophagosome formation—a step essential for autophagosome membrane expansion and cargo sequestration.

Antibodies targeting ATG3 are vital tools in autophagy research, enabling the detection and quantification of ATG3 protein levels in cells or tissues. They are widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to study autophagy dynamics under various conditions, such as nutrient deprivation, stress, or disease states. Researchers also employ these antibodies to investigate ATG3’s interaction partners, post-translational modifications, and regulatory mechanisms.

Dysregulation of ATG3 has been implicated in diseases including cancer, neurodegenerative disorders (e.g., Alzheimer’s and Parkinson’s), and infectious diseases, as autophagy plays dual roles in promoting cell survival or death depending on context. ATG3 antibodies thus aid in exploring its therapeutic potential as a biomarker or drug target. Commercial ATG3 antibodies are typically validated for specificity across species (human, mouse, rat) and are essential for advancing both basic and translational studies in autophagy biology.

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ATG3抗体;ATG3;ATG3 Antibody;

公司简介

湖北瑞和宁生物科技有限公司研究创新能力， 拥有完整、科学、质量管理体系。并不断致力于技术创新，产品创新和管理创新，赢得了国内外广大用户的信任。

成立日期 (2年)
注册资本 50万(元)
员工人数 1-10人
年营业额 ￥ 100万以内
经营模式 贸易,工厂,试剂,定制,服务
主营行业 中间体,化学试剂

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