ADAR抗体

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     Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)    

  
  

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     Western blot analysis using ADAR mouse mAb against Ramos (1), K562 (2), and Jurkat (3) cell lysate.    

  
  

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     Flow cytometric analysis of HeLa cells using ADAR mouse mAb (green) and negative control (red).    

  
  

ADAR (adenosine deaminases acting on RNA) antibodies are essential tools for studying RNA editing, a post-transcriptional process where adenosine (A) in RNA molecules is converted to inosine (I). This editing, catalyzed by ADAR enzymes, alters mRNA coding potential and regulates gene expression. ADARs are critical in diverse biological processes, including neural development, immune response, and cancer progression. Two primary isoforms, ADAR1 and ADAR2. exist in mammals. ADAR1. widely expressed, plays roles in innate immunity by preventing aberrant immune activation against self-RNA, while ADAR2 is predominantly active in the brain, ensuring proper neuronal function. Dysregulation of ADAR activity is linked to diseases such as Aicardi-Goutières syndrome, epilepsy, and malignancies.

ADAR antibodies enable researchers to detect and quantify ADAR protein levels, assess cellular localization (e.g., nuclear vs. cytoplasmic), and investigate functional interactions. They are used in techniques like Western blotting, immunohistochemistry, and immunoprecipitation. Specific antibodies targeting distinct ADAR epitopes or isoforms help dissect their unique roles. For instance, ADAR1-p150 (interferon-inducible) and ADAR1-p110 (constitutive) antibodies differentiate between splice variants. These antibodies also aid in exploring ADAR's involvement in viral infection responses, autoimmune disorders, and RNA-editing-targeted therapies. Validation of antibody specificity remains crucial, as cross-reactivity or off-target binding can lead to misleading conclusions. Overall, ADAR antibodies are indispensable for unraveling the complexity of RNA editing mechanisms and their pathophysiological implications.