ARFGAP1抗体

+ +

•  

   

     Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)    

  
  

•  

   

     Western blot analysis using ARFGAP1 mouse mAb against MOLT4 (1), C2C12 (2), HepG2 (3), MCF-7 (4), and Lncap (5) cell lysate.    

  
  

•  

   

     Flow cytometric analysis of HepG2 cells using ARFGAP1 mouse mAb (green) and negative control (red).    

  
  

ARFGAP1 (ADP-ribosylation factor GTPase-activating protein 1) is a regulatory protein involved in intracellular membrane trafficking, primarily functioning as a GTPase-activating protein (GAP) for ADP-ribosylation factors (ARFs). ARFs are small GTP-binding proteins that cycle between active GTP-bound and inactive GDP-bound states, modulating vesicle formation, cargo sorting, and cytoskeletal dynamics. ARFGAP1 promotes the hydrolysis of GTP to GDP on ARF1. facilitating the dissociation of coat proteins (e.g., COPI) from vesicles during retrograde transport from the Golgi to the endoplasmic reticulum. This process is critical for maintaining Golgi structure, lipid homeostasis, and protein secretion.

Antibodies targeting ARFGAP1 are essential tools for studying its expression, localization, and function in cellular trafficking pathways. They are widely used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to investigate ARFGAP1’s role in physiological processes, including receptor endocytosis, lipid droplet formation, and cell signaling. Dysregulation of ARFGAP1 has been implicated in pathologies such as cancer metastasis and neurodegenerative disorders, underscoring its biomedical relevance. Commercially available ARFGAP1 antibodies are typically raised against specific epitopes (e.g., human ARFGAP1 N-terminal or C-terminal regions) and validated for cross-reactivity in model organisms. Researchers rely on these antibodies to explore mechanistic links between ARFGAP1 activity and disease pathways, aiding drug discovery and diagnostic development.