CDC37抗体

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     Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)    

  
  

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     Western blot analysis using CDC37 mouse mAb against K562 (1), LNcap (2), A431 (3), HEK293 (4), and C2C12 (5) cell lysate.    

  
  

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     Flow cytometric analysis of K562 cells using CDC37 mouse mAb (green) and negative control (red).    

  
  

CDC37. also known as cell division cycle 37 homolog, is a critical co-chaperone that interacts with the heat shock protein 90 (HSP90) to regulate the stability and activation of client proteins, particularly protein kinases. It serves as a kinase-specific adaptor, recruiting nascent or misfolded kinase clients to the HSP90 chaperone machinery for proper folding, maturation, or stabilization. This interaction is essential for maintaining the functional integrity of numerous signaling pathways involved in cell proliferation, survival, and stress responses.

Antibodies targeting CDC37 are widely used in research to investigate its role in HSP90-mediated protein quality control and its association with diseases, including cancer. Overexpression or dysregulation of CDC37 has been implicated in tumorigenesis, as it supports the stability of oncogenic kinases (e.g., AKT, BRAF, CDK4). These antibodies enable the detection of CDC37 expression levels, its interaction with HSP90 or client kinases, and post-translational modifications (e.g., phosphorylation) that modulate its function. Common applications include Western blotting, immunoprecipitation, immunofluorescence, and studying therapeutic strategies aimed at disrupting the CDC37-HSP90 axis.

Researchers often validate CDC37 antibodies for specificity across species (human, mouse, rat) and isoforms. Some antibodies distinguish phosphorylated forms (e.g., at Ser13), which regulate client binding. Their utility extends to cancer models, neurodegenerative disease studies, and exploring chaperone-targeted therapies. Careful selection based on experimental needs (e.g., epitope region, host species) is critical for reliable results.