ADAMTS1抗体

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     Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)    

  
  

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     Western blot analysis using ADAMTS1 mouse mAb against Hela (1) and SK-Br-3 (2) cell lysate.    

  
  

**Background of ADAMTS1 Antibody**

ADAMTS1 (A Disintegrin and Metalloproteinase with Thrombospondin Motifs 1) is a secreted extracellular matrix (ECM)-associated protease belonging to the ADAMTS family. It plays critical roles in tissue remodeling, angiogenesis inhibition, inflammation, and organ development by cleaving components like aggrecan, versican, and thrombospondin. Structurally, ADAMTS1 contains a prodomain, metalloproteinase domain, disintegrin-like module, thrombospondin type-1 repeats (TSR), and a spacer region, which collectively mediate substrate specificity and interactions with ECM proteins.

ADAMTS1 antibodies are essential tools for studying its expression, localization, and function in physiological and pathological contexts, such as cancer, cardiovascular diseases, and osteoarthritis. These antibodies are commonly used in techniques like Western blotting, immunohistochemistry (IHC), and ELISA to detect ADAMTS1 in various biological samples. Researchers often prioritize antibodies validated for specificity through knockout controls or immunoprecipitation, as ADAMTS1 shares structural homology with other ADAMTS members.

Due to its involvement in disease pathways, ADAMTS1 antibodies also aid in exploring therapeutic targets. However, factors like species reactivity (e.g., human, mouse, rat), post-translational modifications (e.g., glycosylation), and epitope accessibility must be considered during antibody selection. Commercial antibodies may vary in clonality (monoclonal vs. polyclonal) and application suitability, necessitating careful validation for experimental reproducibility.