ANAPC10抗体

The ANAPC10 antibody is a tool used to study the Anaphase-Promoting Complex/Cyclosome (APC/C), a multi-subunit E3 ubiquitin ligase critical for cell cycle regulation. ANAPC10 (APC10) is a core subunit of the APC/C, which mediates the ubiquitination of specific target proteins, marking them for proteasomal degradation. This process drives transitions between cell cycle phases, particularly the metaphase-to-anaphase shift and exit from mitosis. ANAPC10 plays a structural role in substrate recognition and coordination with co-activators like CDC20 or CDH1. Antibodies targeting ANAPC10 are widely employed in research to investigate APC/C assembly, activity, and its dysregulation in diseases such as cancer, where cell cycle defects are common. These antibodies enable techniques like Western blotting, immunofluorescence, and immunoprecipitation to assess ANAPC10 expression levels, localization, and interactions within the complex. Studies using ANAPC10 antibodies have contributed to understanding how APC/C dysfunction leads to genomic instability or uncontrolled proliferation. However, specificity and batch variability remain challenges, requiring validation via knockout controls or orthogonal methods. Overall, ANAPC10 antibodies are vital for dissecting the molecular mechanisms of cell cycle control and their implications in health and disease.